Testing of bio-rational and synthetic pesticides to manage cabbage aphid (Brevicoryne brassicae L.) in cabbage field at Rampur, Chitwan, Nepal

Resistance of Cabbage Aphid, Brevicoryne brassicae (Linnaeus) to Endosulfan, Organophosphates and Synthetic Pyrethroids

Laboratory bioassays were done to determine the level of resistance to endosulfan, cypermethrin, deltamethrin, methamidophos, profenofos and chlorpyrifos in field populations of cabbage aphid, Brevicoryne brassicae (L). Comparison between Multan and Kabirwala strains revealed that the level of resistance in Multan strain was comparatively higher to methamidophos, profenofos and chlorpyrifos as ...

Purification and characterisation of a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.).

Plant myrosinases and glucosinolates constitute a defence system in cruciferous plants towards pests and diseases. We have purified for the first time a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.) to homogeneity. The protein was N-terminally blocked and protease (trypsin and lys c) degradation gave peptides of which five were sequenced. The protein is a dimer with sub...

Cytogenetic analysis on the holocentric chromosomes of the cabbage aphid Brevicoryne brassicae

Chromatin organization in the holocentric chromosomes of the aphid Brevicoryne brassicae has been investigated at a cytological level after C-banding, NOR, Giemsa, DAPI and CMA3 staining. C-banding technique showed the presence of heterochromatic bands on both telomeres of the two X chromosomes, whereas only the longest pair of autosomes show distinct intercalary C-positive bands. Moreover, sil...

Purification and characterization of myrosinase from the cabbage aphid (Brevicoryne brassicae), a brassica herbivore.

Aphids are among the most serious insect pests of agricultural crops in the world. They often have specific hosts, and the cabbage aphid (Brevicoryne brassicae) is a specialist on Cruciferae. It has previously been described that certain insects contain the enzyme myrosinase (EC 3.2.3.1), which is considered an important defence enzyme of crucifers. Myrosinase was purified to homogeneity from c...

Evaluation of soil-applied systemic insecticides against cabbage aphid,brevicoryne brassicae and resultant survival of the primary parasitoid diaeretiella rapae on cabbage